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These results indicate that the nucleotides included in Nucleo CMP Forte ® are promising therapeutic molecules for the prevention of neuronal death in brain caused by focal ischemia, Parkinson's disease or other neurodegenerative pathologies. More interestingly, drug pre-treatment significantly reduced MPP + -and glutamate-induced cell death in SH-SY5Y cells and in rat cortical cells. Nucleo CMP Forte ® pre-treatment significantly increased the rate of cell division in SH-SY5Y cells, as well as the synthesis of triglycerides and phospholipids. Cell viability was measured at different times. We used the human dopaminergic cell line SH-SY5Y and a primary culture of rat cortical cells pre-treated with the drug for 24 hours and then exposed to MPP + or glutamate at a range of concentrations.
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We examined its neuroprotective effects on cell toxicity induced by glutamate excitotoxicity or by 1-methyl-4-phenyl-pyridinium (MPP +), an in vitro cell model of Parkinson's disease. Its effects on brain pathologies has received little attention.
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It has been prescribed for peripheral nervous system disorders, such as lumbo-sciatalgia, diabetic or alcoholic polyneuropathy, or trigeminal neuralgia. Nucleo CMP Forte ® is a nucleotide-based drug consisting of cytidinemonophosphate, uridinemonophosphate, uridine-diphosphate and uridinetriphosphate.